Investigation and characterization of RNA-loaded liposome vaccines

Experimental methods and characterisation techniques
We have previously demonstrated that certain combinations of lipids within a liposome formulation enable the highly effective delivery of RNA liposome vaccines to cells. However, such formulations have to date only been prepared at laboratory scale and methods are required whereby they can be manufactured in sufficient quantities for clinical trials […]

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ER2-B: Investigation of membrane permeating materials in biostabilisation

Experimental methods and characterisation techniques in cell preservation and biostabilisation
Evaluate how size, shape and physical nature (net charge, pH responsiveness and nanoparticle and CNT characteristics) affect their interaction with membranes. These studies aim to link transfection efficiency from different therapeutic models (size, charge and proteins versus DNA) with the structural design of the therapeutic nanoparticles, […]

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ESR10-C: Bioengineering of membrane associating polymers and polymer-protein complexes

Investigation of membrane associating polymers, therapeutic nanoparticles and cell preservation experiments
Biotechnology and and bioengineering, experimental
Alex Chen

A safe polymer technology, which can be fine-tuned to deliver a wide variety of payloads into cells for various applications.
Novel pH-responsive, biodegradable polymers will be synthesized to mimic the activity of viruses, both in their cell entry and endosomal escape […]

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Biomolecule Surface Patterning May Enhance Membrane Association

S. Pogodin, N. K. H. Slater and V. A. Baulin

ACS Nano, 6(2), 1308-1313 (2012)

Under dehydration conditions, amphipathic late embryogenesis abundant proteins fold spontaneously from a random conformation into α-helical structures, and this transition is promoted by the presence of membranes. To gain insight into the thermodynamics of membrane association, we model the resulting α-helical structures […]

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Surface patterning of carbon nanotubes can enhance their penetration through a phospholipid bilayer

S. Pogodin, N.K.H. Slater and V.A. Baulin
ACS Nano, 5 (2), 1141–1146 (2011)

Nanotube patterning may occur naturally upon the spontaneous self-assembly of biomolecules onto the surface of single-walled carbon nanotubes (SWNTs). It results in periodically alternating bands of surface properties, ranging from relatively hydrophilic to hydrophobic, along the axis of the nanotube. Single-chain mean field (SCMF) […]

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