Influence of a pH-sensitive polymer on the structure of monoolein cubosomes

Soft Matter, 13, 7571-7577 (2017)
Monika Kluzek, Arwen I. I. Tyler, Shiqi Wang, Rongjun Chen, Carlos M. Marques, Fabrice Thalmann, John M. Seddon, Marc Schmutz
Cubosomes consist in submicron size particles of lipid bicontinuous cubic phases stabilized by surfactant polymers. They provide an appealing road towards the practical use of lipid cubic phases for pharmaceutical and cosmetic […]

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pH-Responsive, Lysine-Based, Hyperbranched Polymers Mimicking Endosomolytic Cell-Penetrating Peptides for Efficient Intracellular Delivery

Chem. Mater., DOI: 10.1021/acs.chemmater.7b00054 (2017).
Shiqi Wang and Rongjun Chen
The insufficient delivery of biomacromolecular therapeutic agents into the cytoplasm of mammalian cells remains a major barrier to their pharmaceutical applications. Cell-penetrating peptides (CPPs) are considered as potential carriers for cytoplasmic delivery of macromolecular drugs. However, due to the positive charge of most CPPs, strong non-specific cell […]

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Membrane-Anchoring, Comb-Like Pseudopeptides for Efficient, pH-Mediated Membrane Destabilization and Intracellular Delivery

ACS Appl. Mater. Interfaces, Article ASAP (2017)
Siyuan Chen, Shiqi Wang, Michal Kopytynski, Marie Bachelet, and Rongjun Chen
Endosomal release has been identified as a rate-limiting step for intracellular delivery of therapeutic agents, in particular macromolecular drugs. Herein, we report a series of synthetic pH-responsive, membrane-anchoring polymers exhibiting dramatic endosomolytic activity for efficient intracellular delivery. The comb-like […]

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Amino Acid Based Hydrogels with Dual Responsiveness for Oral Drug Delivery

Macromol. Biosci. (2016)
Shiqi Wang, Xiaoxue Liu, Ignacio J. Villar-Garcia and Rongjun Chen

This study reports a series of novel amino acid based dual-responsive hydrogels. Prepared by a facile one-pot 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) coupling reaction, the solid content, structure, and mechanical behavior of hydrogels could be easily adjusted by changing the concentrations of the polymers and the crosslinkers. With pH-responsive anionic pseudo-peptides […]

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Imperial College London: Fundamentals and Applications of Lipid Membrane Biophysics

Dates: 22nd-24th September 2015
Location: Horsley Park (Ockham Road South, East Horsley, KT24 6DT)

Lipid membranes are the basic building blocks for almost all living organisms. For years numerous reports have been focused on this area to establish a comprehensive understanding of membrane structures. More recently, the development of modern experimental techniques, such as small-angle X-ray scattering, small-angle neutron scattering and […]

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Gel permeation chromatography/ Size exclusion chromatography

Gel permeation chromatography (GPC)/ Size exclusion chromatography (SEC) is a widely used technique for  measuring the molecular weight and molecular weight distribution of natural and synthetic polymers. It is based on  liquid chromatographic techniques that separate individual polymer chains on the basis of their size in solution and not on their chemical properties. The separation […]

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Nuclear magnetic resonance spectroscopy (NMR spectroscopy)

Nuclear magnetic resonance spectroscopy (NMR spectroscopy) is one of the most widely used techniques by chemists and biologists to identify molecular structures. It relies on the  phenomenon of nuclear magnetic resonance, which means the intramolecular magnetic around an atom in a specific molecular changes the resonance frequency. Thus, different atoms within one molecule could have different resonance […]

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Summary of the Meeting in Cambridge 1 October 2014

1-2 October 2014 – Cambridge, Department of Chemical Engineering at the University of Cambridge.

After months of discussions and proposals conducted by supervisors from each host institution and the program director, Dr. Vladimir Baulin, the management meeting in Cambridge sets the beginning of a great adventure!
In an atmosphere both earnest and casual, many topics have been […]

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ESR5-L: Synthesis and characterization of functional biomimetic polymers for intracellular delivery

Chemical synthesis, characterisation of polymers and nano-objects
This project is focused on development of functional biomimetic polymers which can traverse across cellular membranes to deliver payloads into the cell interior.

Synthesis and characterization of pH-responsive, membrane-active polymers.
Preparation of therapeutic nanoparticles incorporating chemotherapeutic agents or biological molecules.
Understanding and controlling of the trafficking of polymers into a model cellular […]

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