Research

Pheochromocytoma (PC12) Cell Response on Mechanobactericidal Titanium Surfaces

Materials 2018, 11(4), 605 (2018); https://doi.org/10.3390/ma11040605
Jason V. Wandiyanto, Denver Linklater, Pallale G. Tharushi Perera, Anna Orlowska, Vi Khanh Truong, Helmut Thissen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Saulius Juodkazis and Elena P. Ivanova
Titanium is a biocompatible material that is frequently used for making implantable medical devices. Nanoengineering of the surface is the common method for increasing material […]

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The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

Materials, 11(1) 60 (2018)
Anna Orlowska, Pallale Tharushi Perera, Mohammad Al Kobaisi, Andre Dias,
Huu Khuong Duy Nguyen, Shahram Ghanaati, Vladimir A. Baulin, Russell J. Crawford, Elena P. Ivanova
Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively […]

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Sugar-based collagen membrane cross-linking increases barrier capacity of membranes

Clin. Oral Invest.,  (2017)
Po-ju Chia-LaiAnna OrlowskaSarah Al-MaawiAndre DiasYunxin ZhangXuejiu WangNiklas ZenderRobert SaderC. James KirkpatrickShahram Ghanaati
This study examines the permeability and barrier capacity of a sugar cross-linked resorbable collagen membrane ex vivo and in vivo. In an ex vivo study, injectable platelet-rich fibrin (i-PRF), a peripheral blood-derived human leukocyte-and-platelet-rich plasma was used to analyze membrane permeability. […]

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Reduction of the relative centrifugal force influences cell number and growth factor release within injectable PRF-based matrices

J Mater Sci: Mater Med, 28, 188 (2017)
Simon Wend, Alica Kubesch, Anna Orlowska, Sarah Al-Maawi, Niklas Zender, Andre Dias, Richard J. Miron, Robert Sader, Patrick Booms, C. James Kirkpatrick, Joseph Choukroun, Shahram Ghanaati
Platelet rich fibrin (PRF) is a blood concentrate system obtained by centrifugation of peripheral blood. First PRF matrices exhibited solid fibrin scaffold, more recently […]

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In vivo cellular reactions to different biomaterials—Physiological and pathological aspects and their consequences

Seminars in Immunology, DOI: 10.1016/j.smim.2017.06.001 (2017).
Sarah Al-Maawi, Anna Orlowska, Robert Sader, C. James Kirkpatrick, Shahram Ghanaati
Biomaterials are widely used in guided bone regeneration (GBR) and guided tissue regeneration (GTR). After application, there is an interaction between the host immune system and the implanted biomaterial, leading to a biomaterial-specific cellular reaction. The present review focuses on […]

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Adsorption of Human Plasma Albumin and Fibronectin onto Nanostructured Black Silicon Surfaces

Langmuir: 10.1021/acs.langmuir.6b02601 (2016)
Duy H. K. Nguyen, Vy T. H. Pham, Mohammad Al Kobaisi, Chris M. Bhadra, Anna Orlowska, Shahram Ghanaati, Berardo Mario Manzi, Vladimir A Baulin, Saulius Juodkazis, Peter Kingshott, Russell J Crawford, and Elena P. Ivanova

The protein adsorption of two human plasma proteins, albumin (Alb) and fibronectin (Fn) onto synthetic nanostructured bactericidal material, black silicon […]

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“Race for the surface”: eukaryotic cells can win

ACS Appl. Mater. Interfaces: 8(34) 22025-22031 (2016)
Vy T. H. Pham, Vi Khanh Truong, Anna Orlowska, Shahram Ghanaati, Mike Barbeck, Patrick Booms, Alex James Fulcher, Chris M. Bhadra, Ricardas Buividas, Vladimir A Baulin, Charles James Kirkpatrick, Pauline Doran, David E. Mainwaring, Saulius Juodkazis, Russell J Crawford, and Elena P. Ivanova

With an aging population and the consequent […]

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ESR12-F: Toxicity of nano-objects on human cells models

In-vitro experiments on living cells, toxicology studies
Contact
Anna Orlowska
Objectives
Main objective is to compare, validate and corroborate the findings of the interaction of the nano-objects synthesised by the network to actual human cell models with the findings observed with the lipid membrane model systems.
Tasks and methodology
Recruited ESR will obtain experience in toxicity, cell- and molecular biological methodology […]

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